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	title = {Modeling the {Dose} {Response} {Relationship} of {Waterborne} {{Acanthamoeba}},
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}
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	title = {Modeling the Dose Response Relationship of Waterborne Acanthamoeba},
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	abstract = {{In the absence of a definitive immunologic correlate of protection against shigellosis, promising Shigella vaccine candidates have been selected based on their ability to confer resistance against experimental challenge with wild-type Shigella in healthy adult volunteers. A limitation of this model has been the low and often variable attack rate of illness among controls, necessitating repeated inpatient studies to demonstrate statistically significant results. In this study, the Shigella challenge model was modified by using bicarbonate buffer instead of skimmed milk as the delivery vehicle to enhance survival of the ingested challenge inoculum. To determine the ability of the modified model to detect protective efficacy, 11 veteran volunteers (previously challenged with S. flexneri 2a in bicarbonate buffer) and 12 immunologically naive control subjects were challenged with 1.4 {\texttimes} 103 c.f.u. S. flexneri 2a. Shigellosis occurred in 3 veterans and 11 control subjects (27 vs 92\%},
	keywords = {bacillary dysentery, infectivity, vaccines, volunteers},
	issn = {0264-410X},
	doi = {10.1016/0264-410X(95)00102-7},
	url = {https://www.sciencedirect.com/science/article/pii/0264410X95001027},
	author = {Kotloff, Karen L. and Nataro, James P. and Losonsky, Genevieve A. and Wasserman, Steven S. and Hale, Thomas L. and Taylor, David N. and Sadoff, Jerald C. and Levine, Myron M.}
}
@article {k95,
	title = {A modified Shigella volunteer challenge model in which the inoculum is administered with bicarbonate buffer: clinical experience and implications for Shigella infectivity},
	journal = {Vaccine},
	volume = {13},
	number = {16},
	year = {1995},
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	author = {Kotloff, Karen L}
}
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}
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	title = {Mycobacterium avium Subspecies paratuberculosis Cultured from Locally and Commercially Pasteurized Cow{\textquoteright}s Milk in the Czech Republic},
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	author = {Ayele, W Y and Svastova, P}
}
