TY - JOUR
KW - bacillary dysentery
KW - infectivity
KW - vaccines
KW - volunteers
AU - Karen Kotloff
AU - James Nataro
AU - Genevieve Losonsky
AU - Steven Wasserman
AU - Thomas Hale
AU - David Taylor
AU - Jerald Sadoff
AU - Myron Levine
AB - {In the absence of a definitive immunologic correlate of protection against shigellosis, promising Shigella vaccine candidates have been selected based on their ability to confer resistance against experimental challenge with wild-type Shigella in healthy adult volunteers. A limitation of this model has been the low and often variable attack rate of illness among controls, necessitating repeated inpatient studies to demonstrate statistically significant results. In this study, the Shigella challenge model was modified by using bicarbonate buffer instead of skimmed milk as the delivery vehicle to enhance survival of the ingested challenge inoculum. To determine the ability of the modified model to detect protective efficacy, 11 veteran volunteers (previously challenged with S. flexneri 2a in bicarbonate buffer) and 12 immunologically naive control subjects were challenged with 1.4 × 103 c.f.u. S. flexneri 2a. Shigellosis occurred in 3 veterans and 11 control subjects (27 vs 92%
BT - Vaccine
DA - jan
DO - 10.1016/0264-410X(95)00102-7
LA - eng
M1 - 16
N2 - {In the absence of a definitive immunologic correlate of protection against shigellosis, promising Shigella vaccine candidates have been selected based on their ability to confer resistance against experimental challenge with wild-type Shigella in healthy adult volunteers. A limitation of this model has been the low and often variable attack rate of illness among controls, necessitating repeated inpatient studies to demonstrate statistically significant results. In this study, the Shigella challenge model was modified by using bicarbonate buffer instead of skimmed milk as the delivery vehicle to enhance survival of the ingested challenge inoculum. To determine the ability of the modified model to detect protective efficacy, 11 veteran volunteers (previously challenged with S. flexneri 2a in bicarbonate buffer) and 12 immunologically naive control subjects were challenged with 1.4 × 103 c.f.u. S. flexneri 2a. Shigellosis occurred in 3 veterans and 11 control subjects (27 vs 92%
PY - 1995
EP - 1488–1494
T2 - Vaccine
TI - A modified {Shigella} volunteer challenge model in which the inoculum is administered with bicarbonate buffer: clinical experience and implications for {Shigella} infectivity
UR - https://www.sciencedirect.com/science/article/pii/0264410X95001027
VL - 13
SN - 0264-410X
ER -