@article{1050,
  keywords = {characterization, Elizabethkingia miricola, lesions, outbreak, pathological, Quasipaa spinosa},
  author = {Xue Lei and Geng Yi and Kai Wang and Ping OuYang and De Chen and Xiao Huang and Chao Huang and Wei Lai and Zi Zhong and Chang Huo and Ze Yang},
  title = {Elizabethkingia miricola infection in Chinese spiny frog (Quasipaa spinosa)},
  abstract = {<p>Abstract Elizabethkingia miricola is a Gram-negative rod which has been incriminated in severe infections in humans. Recently, a serious infectious disease was identified in Chinese spiny frogs (Quasipaa spinosa), in the Sichuan Province of China; the disease was characterized by corneal opacity, the presence of ascites and neurological symptoms. A Gram-negative bacillus was isolated from the liver, spleen and kidney of the diseased frogs. Experimental infection test revealed that the bacillus could infect the frogs Q.&nbsp;spinosa and the LD50 value was 1.19&nbsp;×&nbsp;106&nbsp;cfu per frog. The isolated Gram-negative bacillus was identified as E.&nbsp;miricola according to phenotypic characteristics, 16S rRNA and gyrB gene sequence analysis. The isolated strain was only susceptible to florfenicol among all investigated chemotherapeutic agents. Histological examination revealed that E.&nbsp;miricola infection caused pathological lesions to multiple organs and tissues, especially in the liver, brain, kidney. These results confirmed that E.&nbsp;miricola is an emerging pathogen of Chinese spiny frogs.</p>
},
  year = {2019},
  journal = {Transboundary and Emerging Diseases},
  volume = {66},
  pages = {1049-1053},
  url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/tbed.13101},
  doi = {https://doi.org/10.1111/tbed.13101},
  language = {eng},
}