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Experiment ID
Acanth_Intranasal2
Agent Strain
A. castellanii HN-3
Contains Preferred Model
No
Reference
Culbertson, C. ., Ensminger, P. ., & Overton, W. . (1966). Hartmannella (acanthamoeba). Experimental chronic, granulomatous brain infections produced by new isolates of low virulence. American Journal of Clinical Pathology, 46, 305–314.
Exposure Route
intranasal
Response
death
Host type
mice
# of Doses
3.00
Dose Units
no of trophozoites
Μodel
exponential
k
1.31E-04
LD50/ID50
5.28E+03
Experiment Dataset
Dose (no. of organsims) Positive Responses Negative Responses Total Subjects/Responses
100 2 78 80
500 4 76 80
1000 10 70 80
Description

Culbertson et al. (1966) studied the pathogenicity of the HN-3 strain of A. castellanii (Culbertson et al., 1966; Marciano-Cabral & Cabral, 2003) on ether-anesthetized-specific-pathogen-free (SPF) mice. Cultures of amebae were grown in trypticase soy broth and diluted so that 0.03 mL of a concentrated suspension could be instilled intranasally into the mice by placing fluid over the nares (Culbertson et al., 1966; Culbertson, Ensminger, & Overton, 1965a; Culbertson, Ensminger, & Overton, 1965b).

The exponential model provided the best fit to the data. 

 

Figure 1. Parameter histogram for exponential model (uncertainty of the parameter)
Figure 1. Parameter histogram for exponential model (uncertainty of the parameter)
Figure 2. Exponential model plot, with confidence bounds around optimized model
Figure 2. Exponential model plot, with confidence bounds around optimized model

Culbertson, C. G., Holmes, D. H., & Overton, W. M. (1965b). Hartmanella castellani (Acanthamoeba sp.). The American Journal of Clinical Pathology, 43(4), 361–364.

Culbertson, C. G., Ensminger, P. W., & Overton, W. M. (1966). Hartmannella (Acanthamoeba): Experimental Chronic, Granulomatous Brain Infections Produced by New Isolates of Low Virulence. The American Journal of Clinical Pathology, 46(3), 305–314.