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	abstract = {Escherichia coli O157:H7 is an emerging food and waterborne pathogen in the U.S. and internationally. The objective of this work was to develop a dose-response model for illness by this organism that bounds the uncertainty in the dose-response relationship. No human clinical trial data are available for E. coli O157:H7, but such data are available for two surrogate pathogens: enteropathogenic E. coli (EPEC) and Shigella dysenteriae. E. coli O157:H7 outbreak data provide an initial estimate of the most likely value of the dose-response relationship within the bounds of an envelope de{\textregistered}ned by beta-Poisson dose-response models {\textregistered}t to the EPEC and S. dysenteriae data. The most likely value of the median effective dose for E. coli O157:H7 is estimated to be approximately 190 000 colony forming units (cfu). At a dose level of 100 cfu, the median response predicted by the model is six percent.},
	author = {Powell, Mark R and Ebel, Eric and Schlosser, Wayne and Walderhaug, Mark and Kause, Janell}
}
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	number = {16},
	year = {1995},
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	author = {Kotloff, Karen L}
}
@article {kotloff_modified_1995,
	title = {A modified {Shigella} volunteer challenge model in which the inoculum is administered with bicarbonate buffer: clinical experience and implications for {Shigella} infectivity},
	journal = {Vaccine},
	volume = {13},
	number = {16},
	year = {1995},
	month = {jan},
	pages = {1488{\textendash}1494},
	abstract = {{In the absence of a definitive immunologic correlate of protection against shigellosis, promising Shigella vaccine candidates have been selected based on their ability to confer resistance against experimental challenge with wild-type Shigella in healthy adult volunteers. A limitation of this model has been the low and often variable attack rate of illness among controls, necessitating repeated inpatient studies to demonstrate statistically significant results. In this study, the Shigella challenge model was modified by using bicarbonate buffer instead of skimmed milk as the delivery vehicle to enhance survival of the ingested challenge inoculum. To determine the ability of the modified model to detect protective efficacy, 11 veteran volunteers (previously challenged with S. flexneri 2a in bicarbonate buffer) and 12 immunologically naive control subjects were challenged with 1.4 {\texttimes} 103 c.f.u. S. flexneri 2a. Shigellosis occurred in 3 veterans and 11 control subjects (27 vs 92\%},
	keywords = {bacillary dysentery, infectivity, vaccines, volunteers},
	issn = {0264-410X},
	doi = {10.1016/0264-410X(95)00102-7},
	url = {https://www.sciencedirect.com/science/article/pii/0264410X95001027},
	author = {Kotloff, Karen L. and Nataro, James P. and Losonsky, Genevieve A. and Wasserman, Steven S. and Hale, Thomas L. and Taylor, David N. and Sadoff, Jerald C. and Levine, Myron M.}
}
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	author = {Ralph R Frerichs and Paul S Keim and Robert Barrais and Renaud Piarroux}
}
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}
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	volume = {285},
	number = {1},
	year = {1971},
	note = {Publisher: Massachusetts Medical Society},
	pages = {1{\textendash}9},
	issn = {0028-4793},
	doi = {10.1056/NEJM197107012850101},
	url = {https://www.nejm.org/doi/10.1056/NEJM197107012850101},
	author = {DuPont, Herbert L. and Formal, Samuel B. and Hornick, Richard B. and Snyder, Merrill J. and Libonati, Joseph P. and Sheahan, Daniel G. and LaBrec, Eugene H. and Kalas, John P.}
}
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