Submitted by kdean on

Experiment ID
Acanth_Cornea1
Agent Strain
Acanthamoeba Ac118
Contains Preferred Model
No
Reference
Badenoch, P. R., Johnson, A. M., Christy, P. E., & Coster, D. J. (1990). Pathogenicity of Acanthamoeba and Corynebacterium in the Rat Cornea. Archives of Ophthalmology, 108, 1. Retrieved from https://jamanetwork.com/journals/jamaophthalmology/article-abstract/638228
Exposure Route
eyes-cornea
Response
infection
Host type
rat
# of Doses
4.00
Dose Units
no of trophozoites
Μodel
exponential
k
1.01E-04
LD50/ID50
6,886
Resampled Parameters
Exponential_Bootstrap.csv
Experiment Dataset
Dose (no. of organisms) Positive Responses Negative Responses Total Subjects/Responses
10 0 8 8
100 0 16 16
1000 2 16 18
10000 5 3 8
Description

Badenoch et al. (1990) studied the combined effect of Acanthamoeba Ac118 (a group III isolate) and the bacterium Corynebacterium xerosis on the corneas of female Porton rats. A constant dose of 104 C. xerosis with increasing doses of Acanthamoeba spp. were injected into incisions in the rat corneas using a microsyringe (Badenoch et al. 1990).

The exponential model provided the best fit to the data. 

Figure 1: Plot of the exponential model fit to Experiment 1 with upper and lower 95% and 99% confidence
Figure 1: Plot of the exponential model fit to Experiment 1 with upper and lower 95% and 99% confidence

 

Figure 2: Histogram of the 10,000 bootstrap replicates of k for the best fitting exponential model fit to Experiment 1
Figure 2: Histogram of the 10,000 bootstrap replicates of k for the best fitting exponential model fit to Experiment 1

 

References

  • Badenoch, P. R., Johnson, A. M., Christy, P. E., & Coster, D. J. (1990). Pathogenicity of Acanthamoeba and Corynebacterium in the Rat Cornea. Archives of Ophthalmology, 108, 1. Retrieved from https://jamanetwork.com/journals/jamaophthalmology/article-abstract/638228