Submitted by admin on

Experiment ID
Acanth_Intranasal4
Pathogen
Acanthamoeba
Agent Strain
A. castellanii HN-3
Contains Preferred Model
No
Reference
Culbertson, C. ., Ensminger, P. ., & Overton, W. . (1966). Hartmannella (acanthamoeba). Experimental chronic, granulomatous brain infections produced by new isolates of low virulence. American Journal of Clinical Pathology, 46, 305–314.
Exposure Route
intranasal
Response
acute meningoencephalitis
Host type
mice
# of Doses
3.00
Dose Units
no of trophozoites
Μodel
exponential
k
2.60E-04
LD50/ID50
2.67E+03
Experiment Dataset
Dose (no. of organisms) Positive Response Negative Response Total Subjects/Responses
100 2 78 80
500 9 71 80
1000 21 59 80
Description

Culbertson et al. (1966) studied the pathogenicity of the HN-3 strain of A. castellanii (Culbertson et al., 1966; Marciano-Cabral & Cabral, 2003) on ether-anesthetized-specific-pathogen-free (SPF) mice. Cultures of amebae were grown in trypticase soy broth and diluted so that 0.03 mL of a concentrated suspension could be instilled intranasally into the mice by placing fluid over the nares (Culbertson et al., 1966; Culbertson, Ensminger, & Overton, 1965a; Culbertson, Ensminger, & Overton, 1965b).

The exponential model provided the best fit to the data. 

Figure 1: Plot of exponential model fit to Experiment 6 with upper and lower 95% and 99% confidence
Figure 1: Plot of exponential model fit to Experiment 6 with upper and lower 95% and 99% confidence
Figure 2: Histogram of the 10,000 bootstrap replicates of k for the best fitting exponential model fit to Experiment 6
Figure 2: Histogram of the 10,000 bootstrap replicates of k for the best fitting exponential model fit to Experiment 6

 

Culbertson, C. G., Ensminger, P. W., & Overton, W. M. (1966). Hartmannella (Acanthamoeba): Experimental Chronic, Granulomatous Brain Infections Produced by New Isolates of Low Virulence. The American Journal of Clinical Pathology, 46(3), 305–314.