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Experiment ID
18,23
Pathogen
Burkholderia mallei
Agent Strain
KHW,316c
Contains Preferred Model
Yes
Reference
Brett, P. J., & Woods, D. E. (1996). Structural and immunological characterization of Burkholderia pseudomallei O-polysaccharide-flagellin protein conjugates. Infection and Immunity, 64, 2824–2828.
Response
death
Host type
C57BL/6 mice and diabetic rat
# of Doses
10.00
Dose Units
CFU
Μodel
beta-Poisson
a
3.28E-01
N50
5.43E+03
LD50/ID50
5.43E+03
Experiment Dataset
Description

Optimization Output for experiment 18 and 23 pooled (B. pseudomallei)

Pooled data of C57BL/6 mice and diabetic rat 
Dose DEATH NOT DEATH Total
150 0 6 6
450 1 5 6
1350 1 5 6
3000 6 4 10
4050 3 3 6
12200 3 3 6
3E+04 7 3 10
3E+05 7 3 10
3E+06 10 0 10
3E+07 10 0 10

 

Goodness of fit and model selection
Model Deviance Δ Degrees 
of freedom		 χ20.95,1 
p-value 		χ20.95,m-k 
p-value
Exponential 63.3 56.6 9 3.84 
5.42e-14 		16.9 
3.15e-10
Beta Poisson 6.68 8 15.5 
0.571
Beta-Poisson fits better than exponential; cannot reject good fit for beta-Poisson.

 

Optimized parameters for the beta-Poisson model, from 10000 bootstrap iterations
Parameter MLE estimate Percentiles
0.5% 2.5% 5% 95% 97.5% 99.5%
α 3.28E-01 1.94E-01 2.16E-01 2.31E-01 5.15E-01 5.86E-01 8.32E-01
N50 5.43E+03 1.82E+03 2.37E+03 2.64E+03 1.23E+04 1.39E+04 1.68E+04
Parameter scatter plot for beta Poisson model ellipses signify the 0.9, 0.95 and 0.99 confidence of the parameters.
Parameter scatter plot for beta Poisson model ellipses signify the 0.9, 0.95 and 0.99 confidence of the parameters.

 

beta Poisson model plot, with confidence bounds around optimized model
beta Poisson model plot, with confidence bounds around optimized model

 

References

  • Liu, B. ., Koo, G. C., Yap, E. H., Chua, K. L., & Y-h, G. . (2002). Model of Differential Susceptibility to Mucosal Burkholderia pseudomallei Infection. Infection and Immunity, 70, 2.