General Overview
Mycobacterium avium subsp. paratuberculosis (MAP) is an obligate pathogenic bacterium of the genus Mycobacterium which causes chronic inflammation of the intestine in domestic and wild ruminants as well as other animals, including primates. M. avium subsp.paratuberculosis can live in animals for years without necessarily causing clinical disease. Infection is widespread in domestic livestock in Europe and North America but can occur elsewhere. [1]
Summary Data
O’Brien et al.(1976) exposed three groups of newly weaned 4monthold red deer orally with M. avium subsp. paratuberculosis Bovine strain and necropsy was conducted 44 weeks post inoculation to determine the infection rate.[2]
Brotherston et al. (1976) inoculated South Country Cheviots at the age of three weeks orally with M. avium subsp. paratuberculosis IOI strain which was originally recovered from a clinical case of the disease in a sheep and VB/4 strain from an affected cow.[3] The necropsy was done 19 months post inoculation.
Recommended Model
Experiment number 262 model is the recommended model. The ID50 of the model has lower value than the other model.
References
 Ayele, W. Y., & Svastova P. (2005). Mycobacterium avium Subspecies paratuberculosis Cultured from Locally and Commercially Pasteurized Cow's Milk in the Czech Republic. Applied and Environmental Microbiology. 71, 12101214.
 O'Brien, R., Mackintosh C. G., Bakker D., Kopecna M., Pavlik I., & Griffin J. F. (2006). Immunological and molecular characterization of susceptibility in relationship to bacterial strain differences in Mycobacterium avium subsp. paratuberculosis infection in the red deer (Cervus elaphus). Infection and Immunity. 74, 3530.
 Brotherston, J. G., Gilmour N. J., & Samuel J. (1961). Quantitative studies of Mycobacterium johnei in the tissues of sheep. Journal of Comparative Pathology. 71, 286.
ID  Exposure Route  # of Doses  Agent Strain  Dose Units  Host type  Μodel  LD50/ID50  Optimized parameters  Response type  Reference 

262  oral  3  sub sp. Paratuberculosis Bovine  CFU  deer  exponential  1000 
k = 6.93E04 
infection  Quantitative studies of Mycobacterium johnei in tissues of sheep. III. Intestinal histopathology." Journal of comparative pathology. 72 (1962): 80.  "
263  oral  3  sub sp. Paratuberculosis IOI strain  CFU  cheviots  betaPoisson  4.8E+02 
a = 5.79E02 N_{50} = 4.8E+02 
infection  Pathogenicity of Acanthamoeba and Corynebacterium in the Rat Cornea." Archives of Ophthalmology. 108 (1990): 1.  "
myco_intravenous  intravenous  5  M. avium serovar 1, human non AIDS isolates: pooled strains N289, N356, N357, N364, N445, N458, N461; Serovar 9 strains N254, N302  CFU  mice  exponential  6.30E+5 
k = 1.10E6 
Lung lesions  
myco_intravenous_2  intravenous  5  MAC 101 (humanderived)  CFU  mice  exponential  2.22E+8 
k = 3.12E9 
death  
myco_intravenous_3  intravenous  15  M. avium serotype II strain SSC 1323 (porcinederived  CFU  pig  exponential  7.49E+7 
k = 9.26E9 
Lesions or culture in lymph nodes  
myco_intravenous_3  intravenous  15  M. avium serotype II strain SSC 1323 (porcinederived)  CFU  pig  exponential  8.79E+6 
k = 7.88E8 
Lesions or culture in lymph nodes  
myco_intravenous_4  intravenous  15  CFU  pig  exponential  4.19E+7 
k = 1.65E8 
Lesions or culture in lymph nodes  
myco_intratracheal  Intratracheal inoculation  3  MAC serotype 8 (Humanderived, AIDS Patient)  CFU  hamsters  betaPoisson  1.42E+8 
a = 0.201 k = 4.87E9 N_{50} = 1.42E+8 
Disseminated infection  
myco_intratracheal_2  Intratracheal inoculation  3  MAC serotype 8 (Humanderived, AIDS Patient)  CFU  hamsters  betaPoisson  7.93E+8 
a = 0.048 N_{50} = 7.93E+8 
Liver infection 
k = 6.93E04
LD_{50}/ID_{50} = 1000



Parameter histogram for Exponential model (uncertainty of the parameter)
Exponential model plot, with confidence bounds around optimized model
References
 O'Brien, R., Mackintosh C. G., Bakker D., Kopecna M., Pavlik I., & Griffin J. F. (2006). Immunological and molecular characterization of susceptibility in relationship to bacterial strain differences in Mycobacterium avium subsp. paratuberculosis infection in the red deer (Cervus elaphus). Infection and Immunity. 74, 3530.
LD_{50}/ID_{50} = 4.8E+02
N_{50} = 4.8E+02



Parameter scatter plot for beta Poisson model ellipses signify the 0.9, 0.95 and 0.99 confidence of the parameters
beta Poisson model plot, with confidence bounds around optimized model
References
 Brotherston, J. G., Gilmour N. J., & Samuel J. (1961). Quantitative studies of Mycobacterium johnei in the tissues of sheep. Journal of Comparative Pathology. 71, 286.
k = 1.10E6
LD_{50}/ID_{50} = 6.30E+5
k = 3.12E9
LD_{50}/ID_{50} = 2.22E+8
k = 9.26E9
LD_{50}/ID_{50} = 7.49E+7
k = 7.88E8
LD_{50}/ID_{50} = 8.79E+6
k = 1.65E8
LD_{50}/ID_{50} = 4.19E+7
k = 4.87E9
LD_{50}/ID_{50} = 1.42E+8
N_{50} = 1.42E+8
LD_{50}/ID_{50} = 7.93E+8
N_{50} = 7.93E+8