General Overview
The famous case of John Snow in London is well known, as an investigation of a cholera outbreak and provided the first in proof of a linkage between the presence of a pathogen in water and a water borne current outbreak. Cholera has remained a major health risk to populations around the world. Vibrio cholerae (V. cholerae) is a gram negative bacterium, the causative agent of cholera, which causes very watery stool, typically referred to ricewatery stool.
Person to person transmission has been typically assumed to be minimal probably due the dose response parameters. However, the main risk posed from this pathogen has always been considered from primary exposure, contaminated water.
http://www.cdc.gov/cholera/index.html
Summary Data
Hornick et al. (1971) has a broad repository of both human and animal data. The most interesting data sets in this study are human exposure where the onset of symptoms as well as indication of asymptomatic infection were monitored.[1] Additionally, both diarrhea and cholera diarrhea (very different in appearance) were also monitored as symptoms.
Cash et al. (1974) has only one dataset which showed a significant trend between dose and observed probability of response. [2] Interestingly, this study determined that in human volunteer feeding trials, the reduction of pH in subject stomachs decreased the overall dose required for a similar response. This was accomplished using sodium bicarbonate, essentially antacid treatment, thus demonstrating the effectiveness of higher pH in some protection from V. cholerea.
Recommended Model
Experiment number 249 is recommended model as it has lowest ID_{50} among the models.
References
 Hornick, R. B., Music S. I., Wenzel R., Cash R., Libonati J. P., Snyder M. J., et al. (1971). The Broad Street pump revisited: response of volunteers to ingested cholera vibrios. Bulletin of the New York Academy of Medicine. 47, 10.
 Cash, R. A., Music S. I., Libonati J. P., Snyder M. J., Wenzel R. P., & Hornick R. B. (1974). Response of Man to Infection with Vibrio cholerae. I. Clinical, Serologic, and Bacteriologic Responses to a Known Inoculum. The Journal of Infectious Diseases. 129, 1.
ID  Exposure Route  # of Doses  Agent Strain  Dose Units  Host type  Μodel  LD50/ID50  Optimized parameters  Response type  Reference 

126  oral (with NaHCO3)  6  Inaba 569B  CFU  human  betaPoisson  6.82E+03 
a = 3.18E01 N_{50} = 6.82E+03 
any diarrhea  Occurrence and toxicological evaluation of cyanobacterial toxins in Alberta lakes and farm dugouts." Water Research. 27 (1993): 3.  "
128  oral (with NaHCO3)  6  Inaba 569B  CFU  human  betaPoisson  3.88E+07 
a = 1.10E01 N_{50} = 3.88E+07 
cholera diarrhea  Experimental Human Salmonellosis: I. Pathogenicity of Strains of Salmonella Meleagridis and Salmonella Anatum Obtained from SprayDried Whole Egg." Oxford Journal of Infectious Diseases. 88.3 (1951).  "
167  oral (no NaHCO3)  7  Inaba 569B (classical)  CFU  human  betaPoisson  2.91E+09 
a = 1.31E01 N_{50} = 2.91E+09 
diarrhea and culture positive  Reactogenicity, immunogenicity and efficacy studies of Escherichia coli type 1 somatic pili parenteral vaccine in man." Scandinavian journal of infectious diseases. Supplementum. 33 (1982): 8395.  "
249  oral (with NaHCO3)  6  Inaba 569B  CFU  human  betaPoisson  2.43E+02 
a = 2.50E01 N_{50} = 2.43E+02 
infection  Effect of repeated oral infection of hamsters with scrapie." Journal of General Virology. 79 (1998).  "
35  oral (no NaHCO3)  7  Inaba 569B  CFU  human  betaPoisson  6.36E+08 
a = 1.98E01 N_{50} = 6.36E+08 
diarrhea or culture positive  The Broad Street pump revisited: response of volunteers to ingested cholera vibrios." Bulletin of the New York Academy of Medicine. 47 (1971): 10.  "
LD_{50}/ID_{50} = 6.82E+03
N_{50} = 6.82E+03



Parameter scatter plot for beta Poisson model ellipses signify the 0.9, 0.95 and 0.99 confidence of the parameters.
beta Poisson model plot, with confidence bounds around optimized model
References
 Hornick, R. B., Music S. I., Wenzel R., Cash R., Libonati J. P., Snyder M. J., et al. (1971). The Broad Street pump revisited: response of volunteers to ingested cholera vibrios. Bulletin of the New York Academy of Medicine. 47, 10.
LD_{50}/ID_{50} = 3.88E+07
N_{50} = 3.88E+07



Parameter scatter plot for beta Poisson model ellipses signify the 0.9, 0.95 and 0.99 confidence of the parameters.
beta Poisson model plot, with confidence bounds around optimized model
References
 Hornick, R. B., Music S. I., Wenzel R., Cash R., Libonati J. P., Snyder M. J., et al. (1971). The Broad Street pump revisited: response of volunteers to ingested cholera vibrios. Bulletin of the New York Academy of Medicine. 47, 10.
LD_{50}/ID_{50} = 2.91E+09
N_{50} = 2.91E+09



Parameter scatter plot for beta Poisson model ellipses signify the 0.9, 0.95 and 0.99 confidence of the parameters.
beta Poisson model plot, with confidence bounds around optimized model
References
 Cash, R. A., Music S. I., Libonati J. P., Snyder M. J., Wenzel R. P., & Hornick R. B. (1974). Response of Man to Infection with Vibrio cholerae. I. Clinical, Serologic, and Bacteriologic Responses to a Known Inoculum. The Journal of Infectious Diseases. 129, 1.
LD_{50}/ID_{50} = 2.43E+02
N_{50} = 2.43E+02



Parameter scatter plot for beta Poisson model ellipses signify the 0.9, 0.95 and 0.99 confidence of the parameters.
beta Poisson model plot, with confidence bounds around optimized model
References
 Hornick, R. B., Music S. I., Wenzel R., Cash R., Libonati J. P., Snyder M. J., et al. (1971). The Broad Street pump revisited: response of volunteers to ingested cholera vibrios. Bulletin of the New York Academy of Medicine. 47, 10.
LD_{50}/ID_{50} = 6.36E+08
N_{50} = 6.36E+08
Please Note
The results from this experiment are not recommended for use, rather they are present for reference purposes. The confidence intervals are not complete for this experiment since the data is not allowing for a stable bootstrap outcome. It is recommended to use the recommended model as this data is likely just capable of passing the test of trend, but still likely suboptimal for dose response modeling purposes.



Parameter scatter plot for beta Poisson model ellipses signify the 0.9, 0.95 and 0.99 confidence of the parameters.
beta Poisson model plot, with confidence bounds around optimized model
References
 Hornick, R. B., Music S. I., Wenzel R., Cash R., Libonati J. P., Snyder M. J., et al. (1971). The Broad Street pump revisited: response of volunteers to ingested cholera vibrios. Bulletin of the New York Academy of Medicine. 47, 10.