Description

Badenoch et al. (1990) studied the combined effect of Acanthamoeba Ac118 (a group III isolate) and the bacterium Corynebacterium xerosis on the corneas of female Portion rats. A constant dose of 106 C. xerosis with increasing doses of Acanthamoeba spp. were injected into incisions in the rat corneas using a microsyringe.

The exponential model provided the best fit to the data.

Figure 1. Plot of exponential model fit to Experiment 2 with upper and lower 95% and 99% confidence
Figure 1. Plot of exponential model fit to Experiment 2 with upper and lower 95% and 99% confidence
Figure 2. Histogram of the 10,000 bootstrap replicates of k for the best fitting exponential model fit to Experiment 2
Figure 2. Histogram of the 10,000 bootstrap replicates of k for the best fitting exponential model fit to Experiment 2
# of Doses
4.00
Μodel
exponential
LD50/ID50
1.91E+03
Dose Units
no of trophozoites
Response
infection
Exposure Route
eyes-cornea
Contains Preferred Model
Status
fitted
k
3.63E-04
Agent Strain
Acanthamoeba Ac118
Experiment ID
Acanth_Cornea2
Host type
rat
Experiment Dataset
Dose (no. of organisms) Positive Response Negative Response Total Subjects/Responses
10 0 8 8
100 0 16 16
1000 7 17 24
10000 10 0 10

Acanthamoeba: Dose Response Experiments (Cornea)

Submitted by kdean on

General overview

Acanthamoeba spp. are free-living amoeba (FLA) that have been commonly found in freshwater, tap water, and recreational water. Acanthamoeba spp.

ID Exposure Route # of Doses Agent Strain Dose Units Host type Μodel LD50/ID50 Optimized parameters Response type Reference
Acanth_Cornea1 eyes-cornea 4.00 Acanthamoeba Ac118 no of trophozoites rat exponential 6,886 k = 1.01E-04 infection
Badenoch, P. R., Johnson, A. M., Christy, P. E., & Coster, D. J. (1990). Pathogenicity of Acanthamoeba and Corynebacterium in the Rat Cornea. Archives of Ophthalmology, 108, 1. Retrieved from https://jamanetwork.com/journals/jamaophthalmology/article-abstract/638228
Acanth_Cornea2 eyes-cornea 4.00 Acanthamoeba Ac118 no of trophozoites rat exponential 1.91E+03 k = 3.63E-04 infection
Badenoch, P. R., Johnson, A. M., Christy, P. E., & Coster, D. J. (1990). Pathogenicity of Acanthamoeba and Corynebacterium in the Rat Cornea. Archives of Ophthalmology, 108, 1. Retrieved from https://jamanetwork.com/journals/jamaophthalmology/article-abstract/638228
Exposure Route:
eyes-cornea
# of Doses:
4.00
Agent Strain:
Acanthamoeba Ac118
Dose Units:
no of trophozoites
Host type:
rat
Μodel:
exponential
LD50/ID50:
6,886
Optimized parameters: k = 1.01E-04
Response type:
infection
Reference:
Pathogenicity of Acanthamoeba and Corynebacterium in the Rat Cornea

Badenoch et al. (1990) studied the combined effect of Acanthamoeba Ac118 (a group III isolate) and the bacterium Corynebacterium xerosis on the corneas of female Porton rats. A constant dose of 104 C. xerosis with increasing doses of Acanthamoeba spp. were injected into incisions in the rat corneas using a microsyringe (Badenoch et al. 1990).

The exponential model provided the best fit to the data. 

Figure 1: Plot of the exponential model fit to Experiment 1 with upper and lower 95% and 99% confidence
Figure 1: Plot of the exponential model fit to Experiment 1 with upper and lower 95% and 99% confidence

 

Figure 2: Histogram of the 10,000 bootstrap replicates of k for the best fitting exponential model fit to Experiment 1
Figure 2: Histogram of the 10,000 bootstrap replicates of k for the best fitting exponential model fit to Experiment 1

 

Exposure Route:
eyes-cornea
# of Doses:
4.00
Agent Strain:
Acanthamoeba Ac118
Dose Units:
no of trophozoites
Host type:
rat
Μodel:
exponential
LD50/ID50:
1.91E+03
Optimized parameters: k = 3.63E-04
Response type:
infection
Reference:
Pathogenicity of Acanthamoeba and Corynebacterium in the Rat Cornea

Badenoch et al. (1990) studied the combined effect of Acanthamoeba Ac118 (a group III isolate) and the bacterium Corynebacterium xerosis on the corneas of female Portion rats. A constant dose of 106 C. xerosis with increasing doses of Acanthamoeba spp. were injected into incisions in the rat corneas using a microsyringe.

The exponential model provided the best fit to the data.

Figure 1. Plot of exponential model fit to Experiment 2 with upper and lower 95% and 99% confidence
Figure 1. Plot of exponential model fit to Experiment 2 with upper and lower 95% and 99% confidence
Figure 2. Histogram of the 10,000 bootstrap replicates of k for the best fitting exponential model fit to Experiment 2
Figure 2. Histogram of the 10,000 bootstrap replicates of k for the best fitting exponential model fit to Experiment 2
Description

Badenoch et al. (1990) studied the combined effect of Acanthamoeba Ac118 (a group III isolate) and the bacterium Corynebacterium xerosis on the corneas of female Porton rats. A constant dose of 104 C. xerosis with increasing doses of Acanthamoeba spp. were injected into incisions in the rat corneas using a microsyringe (Badenoch et al. 1990).

The exponential model provided the best fit to the data. 

Figure 1: Plot of the exponential model fit to Experiment 1 with upper and lower 95% and 99% confidence
Figure 1: Plot of the exponential model fit to Experiment 1 with upper and lower 95% and 99% confidence

 

Figure 2: Histogram of the 10,000 bootstrap replicates of k for the best fitting exponential model fit to Experiment 1
Figure 2: Histogram of the 10,000 bootstrap replicates of k for the best fitting exponential model fit to Experiment 1

 

# of Doses
4.00
Μodel
exponential
LD50/ID50
6,886
Dose Units
no of trophozoites
Response
infection
Exposure Route
eyes-cornea
Contains Preferred Model
Status
fitted
Resampled Parameters
Exponential_Bootstrap.csv
k
1.01E-04
Agent Strain
Acanthamoeba Ac118
Experiment ID
Acanth_Cornea1
Host type
rat
Experiment Dataset
Dose (no. of organisms) Positive Responses Negative Responses Total Subjects/Responses
10 0 8 8
100 0 16 16
1000 2 16 18
10000 5 3 8
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