\[P(response)=1-exp(-k\times dose)\]

Description

Culbertson et al. (1966) studied the pathogenicity of the HN-3 strain of A. castellanii (Culbertson et al., 1966; Marciano-Cabral & Cabral, 2003) on ether-anesthetized-specific-pathogen-free (SPF) mice. Cultures of amebae were grown in trypticase soy broth and diluted so that 0.03 mL of a concentrated suspension could be instilled intranasally into the mice by placing fluid over the nares (Culbertson et al., 1966; Culbertson, Ensminger, & Overton, 1965a; Culbertson, Ensminger, & Overton, 1965b).

The exponential model provided the best fit to the data. 

Figure 1: Plot of exponential model fit to Experiment 6 with upper and lower 95% and 99% confidence
Figure 1: Plot of exponential model fit to Experiment 6 with upper and lower 95% and 99% confidence
Figure 2: Histogram of the 10,000 bootstrap replicates of k for the best fitting exponential model fit to Experiment 6
Figure 2: Histogram of the 10,000 bootstrap replicates of k for the best fitting exponential model fit to Experiment 6

 

Culbertson, C. G., Ensminger, P. W., & Overton, W. M. (1966). Hartmannella (Acanthamoeba): Experimental Chronic, Granulomatous Brain Infections Produced by New Isolates of Low Virulence. The American Journal of Clinical Pathology, 46(3), 305–314.

# of Doses
3.00
Μodel
exponential
LD50/ID50
2.67E+03
Dose Units
no of trophozoites
Response
acute meningoencephalitis
Exposure Route
intranasal
Contains Preferred Model
Status
fitted
k
2.60E-04
Agent Strain
A. castellanii HN-3
Experiment ID
Acanth_Intranasal4
Host type
mice
Experiment Dataset
Dose (no. of organisms) Positive Response Negative Response Total Subjects/Responses
100 2 78 80
500 9 71 80
1000 21 59 80
Description

Culbertson et al. (1966) studied the pathogenicity of the HN-3 strain of A. castellanii (Culbertson et al., 1966; Marciano-Cabral & Cabral, 2003) on ether-anesthetized-specific-pathogen-free (SPF) mice. Cultures of amebae were grown in trypticase soy broth and diluted so that 0.03 mL of a concentrated suspension could be instilled intranasally into the mice by placing fluid over the nares (Culbertson et al., 1966; Culbertson, Ensminger, & Overton, 1965a; Culbertson, Ensminger, & Overton, 1965b).

The exponential model provided the best fit to the data. 

Figure 1: Plot of exponential model fit to Experiment 5 with upper and lower 95% and 99% confidence
Figure 1: Plot of exponential model fit to Experiment 5 with upper and lower 95% and 99% confidence

Culbertson, C. G., Holmes, D. H., & Overton, W. M. (1965b). Hartmanella castellani (Acanthamoeba sp.). The American Journal of Clinical Pathology, 43(4), 361–364.

Culbertson, C. G., Ensminger, P. W., & Overton, W. M. (1966). Hartmannella (Acanthamoeba): Experimental Chronic, Granulomatous Brain Infections Produced by New Isolates of Low Virulence. The American Journal of Clinical Pathology, 46(3), 305–314.

Figure 2: Histogram of the 10,000 bootstrap replicates of k for the best fitting exponential model fit to Experiment 5
Figure 2: Histogram of the 10,000 bootstrap replicates of k for the best fitting exponential model fit to Experiment 5
# of Doses
3.00
Μodel
exponential
LD50/ID50
749
Dose Units
no of trophozoites
Response
brain invasion
Exposure Route
intranasal
Contains Preferred Model
Status
fitted
k
9.26E-04
Agent Strain
A. castellanii HN-3
Experiment ID
Acanth_Intranasal3
Host type
mice
Experiment Dataset
Dose (no. of organisms) Positive Response Negative Response Total Subjects/Responses
100 7 73 80
500 31 49 80
1000 43 37 80
Description

Culbertson et al. (1966) studied the pathogenicity of the HN-3 strain of A. castellanii (Culbertson et al., 1966; Marciano-Cabral & Cabral, 2003) on ether-anesthetized-specific-pathogen-free (SPF) mice. Cultures of amebae were grown in trypticase soy broth and diluted so that 0.03 mL of a concentrated suspension could be instilled intranasally into the mice by placing fluid over the nares (Culbertson et al., 1966; Culbertson, Ensminger, & Overton, 1965a; Culbertson, Ensminger, & Overton, 1965b).

The exponential model provided the best fit to the data. 

 

Figure 1. Parameter histogram for exponential model (uncertainty of the parameter)
Figure 1. Parameter histogram for exponential model (uncertainty of the parameter)
Figure 2. Exponential model plot, with confidence bounds around optimized model
Figure 2. Exponential model plot, with confidence bounds around optimized model

Culbertson, C. G., Holmes, D. H., & Overton, W. M. (1965b). Hartmanella castellani (Acanthamoeba sp.). The American Journal of Clinical Pathology, 43(4), 361–364.

Culbertson, C. G., Ensminger, P. W., & Overton, W. M. (1966). Hartmannella (Acanthamoeba): Experimental Chronic, Granulomatous Brain Infections Produced by New Isolates of Low Virulence. The American Journal of Clinical Pathology, 46(3), 305–314.

# of Doses
3.00
Μodel
exponential
LD50/ID50
5.28E+03
Dose Units
no of trophozoites
Response
death
Exposure Route
intranasal
Contains Preferred Model
Status
fitted
k
1.31E-04
Agent Strain
A. castellanii HN-3
Experiment ID
Acanth_Intranasal2
Host type
mice
Experiment Dataset
Dose (no. of organsims) Positive Responses Negative Responses Total Subjects/Responses
100 2 78 80
500 4 76 80
1000 10 70 80
Description

Badenoch et al. (1990) studied the combined effect of Acanthamoeba Ac118 (a group III isolate) and the bacterium Corynebacterium xerosis on the corneas of female Portion rats. A constant dose of 106 C. xerosis with increasing doses of Acanthamoeba spp. were injected into incisions in the rat corneas using a microsyringe.

The exponential model provided the best fit to the data.

Figure 1. Plot of exponential model fit to Experiment 2 with upper and lower 95% and 99% confidence
Figure 1. Plot of exponential model fit to Experiment 2 with upper and lower 95% and 99% confidence
Figure 2. Histogram of the 10,000 bootstrap replicates of k for the best fitting exponential model fit to Experiment 2
Figure 2. Histogram of the 10,000 bootstrap replicates of k for the best fitting exponential model fit to Experiment 2
# of Doses
4.00
Μodel
exponential
LD50/ID50
1.91E+03
Dose Units
no of trophozoites
Response
infection
Exposure Route
eyes-cornea
Contains Preferred Model
Status
fitted
k
3.63E-04
Agent Strain
Acanthamoeba Ac118
Experiment ID
Acanth_Cornea2
Host type
rat
Experiment Dataset
Dose (no. of organisms) Positive Response Negative Response Total Subjects/Responses
10 0 8 8
100 0 16 16
1000 7 17 24
10000 10 0 10
Description
 
Host
Pig/ 8-10 weeks old
# of Doses
15.00
Μodel
exponential
LD50/ID50
4.19E+7
Dose Units
CFU
Response
Lesions or culture in lymph nodes
Exposure Route
intravenous
Contains Preferred Model
Status
fitted
k
1.65E-8
Experiment ID
myco_intravenous_4
Host type
pig
Description
Host
Pig/ 8-10 weeks old
# of Doses
15.00
Μodel
exponential
LD50/ID50
8.79E+6
Dose Units
CFU
Response
Lesions or culture in lymph nodes
Exposure Route
intravenous
Contains Preferred Model
Status
fitted
k
7.88E-8
Agent Strain
M. avium serotype II strain SSC 1323 (porcine-derived)
Experiment ID
myco_intravenous_3
Host type
pig
Description
myco_3.png
Host
Pig/ 8-10 weeks old
# of Doses
15.00
Μodel
exponential
LD50/ID50
7.49E+7
Dose Units
CFU
Response
Lesions or culture in lymph nodes
Exposure Route
intravenous
Contains Preferred Model
Status
fitted
k
9.26E-9
Agent Strain
M. avium serotype II strain SSC 1323 (porcine-derived
Experiment ID
myco_intravenous_3
Host type
pig
Experiment Dataset
Description
myco
Host
C57BL/6 beige mice/NS
# of Doses
5.00
Μodel
exponential
LD50/ID50
2.22E+8
Dose Units
CFU
Response
death
Exposure Route
intravenous
Contains Preferred Model
Status
fitted
k
3.12E-9
Agent Strain
MAC 101 (human-derived)
Experiment ID
myco_intravenous_2
Host type
mice
Experiment Dataset
Description
Mycobacterium_Avium_1
Host
Female C57BL/6 beige Mice/5 weeks
# of Doses
5.00
Μodel
exponential
LD50/ID50
6.30E+5
Dose Units
CFU
Response
Lung lesions
Exposure Route
intravenous
Contains Preferred Model
Status
fitted
k
1.10E-6
Agent Strain
M. avium serovar 1, human non- AIDS isolates: pooled strains N-289, N-356, N-357, N-364, N-445, N458, N-461; Serovar 9 strains N-254, N-302
Experiment ID
myco_intravenous
Host type
mice
Experiment Dataset
Description
Intraperitoneal2.png
Host
Balb/c Mice
# of Doses
4.00
Μodel
exponential
LD50/ID50
4.23E+08
Dose Units
CFU
Response
death
Exposure Route
intraperitoneal
Contains Preferred Model
Status
fitted
k
1.64E-09
Agent Strain
E. anopheles
Experiment ID
ek_ intraperitoneal2
Experiment Dataset
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