Description

Červa (1967a,b) studied white mice of the Czechoslovak H-strain weighing 13-15 grams inoculated intranasally by placing 0.02 mL of the A1 strain of Acanthamoeba over the nares of the ethyl-ether anesthetized mice (Cerva, 1967b).

The beta-Poisson model provided the best fit to the data. 

 

Cerva, L. (1967b). Intranasal, Intrapulmonary, and Intracardial Inoculation of Experimental Animals with Hartmanella castellanii. Folia Parasitologica (Praha), 14, 207–215.

# of Doses
6.00
Μodel
beta-Poisson
N50
14,690 or 14,538
Dose Units
no of trophozoites
Response
death
Exposure Route
intranasal
Contains Preferred Model
Status
fitted
a
0.161
Agent Strain
A. culbertsoni (A1)
Experiment ID
Acanth_Intranasal1
Host type
mice
Experiment Dataset
Dose (no. of organisms) Positive Response Negative Response Total Subjects/Responses
3 0 20 20
30 2 18 20
300 1 19 20
3000 9 11 20
Description

Culbertson et al. (1966) studied the pathogenicity of the HN-3 strain of A. castellanii (Culbertson et al., 1966; Marciano-Cabral & Cabral, 2003) on ether-anesthetized-specific-pathogen-free (SPF) mice. Cultures of amebae were grown in trypticase soy broth and diluted so that 0.03 mL of a concentrated suspension could be instilled intranasally into the mice by placing fluid over the nares (Culbertson et al., 1966; Culbertson, Ensminger, & Overton, 1965a; Culbertson, Ensminger, & Overton, 1965b).

The exponential model provided the best fit to the data. 

Figure 1: Plot of exponential model fit to Experiment 6 with upper and lower 95% and 99% confidence
Figure 1: Plot of exponential model fit to Experiment 6 with upper and lower 95% and 99% confidence
Figure 2: Histogram of the 10,000 bootstrap replicates of k for the best fitting exponential model fit to Experiment 6
Figure 2: Histogram of the 10,000 bootstrap replicates of k for the best fitting exponential model fit to Experiment 6

 

Culbertson, C. G., Ensminger, P. W., & Overton, W. M. (1966). Hartmannella (Acanthamoeba): Experimental Chronic, Granulomatous Brain Infections Produced by New Isolates of Low Virulence. The American Journal of Clinical Pathology, 46(3), 305–314.

# of Doses
3.00
Μodel
exponential
LD50/ID50
2.67E+03
Dose Units
no of trophozoites
Response
acute meningoencephalitis
Exposure Route
intranasal
Contains Preferred Model
Status
fitted
k
2.60E-04
Agent Strain
A. castellanii HN-3
Experiment ID
Acanth_Intranasal4
Host type
mice
Experiment Dataset
Dose (no. of organisms) Positive Response Negative Response Total Subjects/Responses
100 2 78 80
500 9 71 80
1000 21 59 80
Description

Culbertson et al. (1966) studied the pathogenicity of the HN-3 strain of A. castellanii (Culbertson et al., 1966; Marciano-Cabral & Cabral, 2003) on ether-anesthetized-specific-pathogen-free (SPF) mice. Cultures of amebae were grown in trypticase soy broth and diluted so that 0.03 mL of a concentrated suspension could be instilled intranasally into the mice by placing fluid over the nares (Culbertson et al., 1966; Culbertson, Ensminger, & Overton, 1965a; Culbertson, Ensminger, & Overton, 1965b).

The exponential model provided the best fit to the data. 

Figure 1: Plot of exponential model fit to Experiment 5 with upper and lower 95% and 99% confidence
Figure 1: Plot of exponential model fit to Experiment 5 with upper and lower 95% and 99% confidence

Culbertson, C. G., Holmes, D. H., & Overton, W. M. (1965b). Hartmanella castellani (Acanthamoeba sp.). The American Journal of Clinical Pathology, 43(4), 361–364.

Culbertson, C. G., Ensminger, P. W., & Overton, W. M. (1966). Hartmannella (Acanthamoeba): Experimental Chronic, Granulomatous Brain Infections Produced by New Isolates of Low Virulence. The American Journal of Clinical Pathology, 46(3), 305–314.

Figure 2: Histogram of the 10,000 bootstrap replicates of k for the best fitting exponential model fit to Experiment 5
Figure 2: Histogram of the 10,000 bootstrap replicates of k for the best fitting exponential model fit to Experiment 5
# of Doses
3.00
Μodel
exponential
LD50/ID50
749
Dose Units
no of trophozoites
Response
brain invasion
Exposure Route
intranasal
Contains Preferred Model
Status
fitted
k
9.26E-04
Agent Strain
A. castellanii HN-3
Experiment ID
Acanth_Intranasal3
Host type
mice
Experiment Dataset
Dose (no. of organisms) Positive Response Negative Response Total Subjects/Responses
100 7 73 80
500 31 49 80
1000 43 37 80
Description

Culbertson et al. (1966) studied the pathogenicity of the HN-3 strain of A. castellanii (Culbertson et al., 1966; Marciano-Cabral & Cabral, 2003) on ether-anesthetized-specific-pathogen-free (SPF) mice. Cultures of amebae were grown in trypticase soy broth and diluted so that 0.03 mL of a concentrated suspension could be instilled intranasally into the mice by placing fluid over the nares (Culbertson et al., 1966; Culbertson, Ensminger, & Overton, 1965a; Culbertson, Ensminger, & Overton, 1965b).

The exponential model provided the best fit to the data. 

 

Figure 1. Parameter histogram for exponential model (uncertainty of the parameter)
Figure 1. Parameter histogram for exponential model (uncertainty of the parameter)
Figure 2. Exponential model plot, with confidence bounds around optimized model
Figure 2. Exponential model plot, with confidence bounds around optimized model

Culbertson, C. G., Holmes, D. H., & Overton, W. M. (1965b). Hartmanella castellani (Acanthamoeba sp.). The American Journal of Clinical Pathology, 43(4), 361–364.

Culbertson, C. G., Ensminger, P. W., & Overton, W. M. (1966). Hartmannella (Acanthamoeba): Experimental Chronic, Granulomatous Brain Infections Produced by New Isolates of Low Virulence. The American Journal of Clinical Pathology, 46(3), 305–314.

# of Doses
3.00
Μodel
exponential
LD50/ID50
5.28E+03
Dose Units
no of trophozoites
Response
death
Exposure Route
intranasal
Contains Preferred Model
Status
fitted
k
1.31E-04
Agent Strain
A. castellanii HN-3
Experiment ID
Acanth_Intranasal2
Host type
mice
Experiment Dataset
Dose (no. of organsims) Positive Responses Negative Responses Total Subjects/Responses
100 2 78 80
500 4 76 80
1000 10 70 80
Description

Badenoch et al. (1990) studied the combined effect of Acanthamoeba Ac118 (a group III isolate) and the bacterium Corynebacterium xerosis on the corneas of female Portion rats. A constant dose of 106 C. xerosis with increasing doses of Acanthamoeba spp. were injected into incisions in the rat corneas using a microsyringe.

The exponential model provided the best fit to the data.

Figure 1. Plot of exponential model fit to Experiment 2 with upper and lower 95% and 99% confidence
Figure 1. Plot of exponential model fit to Experiment 2 with upper and lower 95% and 99% confidence
Figure 2. Histogram of the 10,000 bootstrap replicates of k for the best fitting exponential model fit to Experiment 2
Figure 2. Histogram of the 10,000 bootstrap replicates of k for the best fitting exponential model fit to Experiment 2
# of Doses
4.00
Μodel
exponential
LD50/ID50
1.91E+03
Dose Units
no of trophozoites
Response
infection
Exposure Route
eyes-cornea
Contains Preferred Model
Status
fitted
k
3.63E-04
Agent Strain
Acanthamoeba Ac118
Experiment ID
Acanth_Cornea2
Host type
rat
Experiment Dataset
Dose (no. of organisms) Positive Response Negative Response Total Subjects/Responses
10 0 8 8
100 0 16 16
1000 7 17 24
10000 10 0 10
Description
Mice/Naegleria fowleri LEE strain data
Dose Dead Survived Total
1E+02 0 10 10
1E+02 0 10 10
1E+02 0 10 10
1E+03 0 10 10
1E+03 1 9 10
1E+03 1 9 10
1E+04 1 9 10
1E+04 4 6 10
1E+04 4 6 10
1E+05 4 6 10
1E+05 6 4 10
1E+05 7 3 10
1E+06 7 3 10

 

Goodness of fit for pooled data
Model Deviance Degrees 
of freedom

χ20.95,1

 

 χ20.95,m-k 
 
Beta Poisson 7.4 11 19.68 0.77
Plot of beta-Poisson model for pooled data of CD1 mice swimming for 5, 10, and 20 minutes with upper and lower 95% and 99% confidence.
Plot of beta-Poisson model for pooled data of CD1 mice swimming for 5, 10, and 20 minutes with upper and lower 95% and 99% confidence.
Bootstrapped distribution of beta-Poisson parameter estimates for pooled data of CD1 mice swimming for 5, 10, and 20 minutes; the center marker (X) represents the maximum likelihood estimate.
Bootstrapped distribution of beta-Poisson parameter estimates for pooled data of CD1 mice swimming for 5, 10, and 20 minutes; the center marker (X) represents the maximum likelihood estimate.

 

 
Host
CD1 Mice
# of Doses
13.00
Μodel
beta-Poisson
N50
5.79E+04
LD50/ID50
5.79E+04
Dose Units
no of trophozoites
Response
death
Exposure Route
Swimming for 5
10 or 20 minutes
Contains Preferred Model
Status
pooled
a
0.226
Experiment ID
954,955,956,957
Experiment Dataset
Description
Mice/Naegleria fowleri LEE strain data
Dose Dead Survived Total
1E+02 0 10 10
1E+03 1 9 10
1E+04 4 6 10
1E+05 7 3 10

 

Goodness of fit and model selection
Model Deviance Δ Degrees 
of freedom

χ20.95,1

 

 χ20.95,m-k 
 
Exponential 6.13 5.90 3 3.84 7.81
Beta Poisson 0.23 2 5.99
Beta-Poisson is preferred to Exponential;
Naegleria fowleri infection acquired by mice through swimming in amebae-contaminated water
Host
CD1 Mice
# of Doses
4.00
Μodel
beta-Poisson
N50
1.98E+04
LD50/ID50
1.98E+04
Dose Units
no of trophozoites
Response
death
Exposure Route
Swimming for 20 minutes
Contains Preferred Model
Status
fitted
a
0.35
Agent Strain
LEE
Experiment ID
957
Experiment Dataset
Description
Mice/Naegleria fowleri LEE strain data
Dose Dead Survived Total
1E+02 0 10 10
1E+03 1 9 10
1E+04 4 6 10
1E+05 6 4 10

 

Goodness of fit and model selection
Model Deviance Δ Degrees 
of freedom

χ20.95,1

 

 χ20.95,m-k 
 
Exponential 7.89 7.55 3 3.84 7.81
Beta Poisson 0.33 2 5.99
Beta-Poisson is preferred to Exponential;

 

Beta-Poisson model plot, with confidence bounds around optimized model
Beta-Poisson model plot, with confidence bounds around optimized model

 

Host
CD1 Mice
# of Doses
4.00
Μodel
beta-Poisson
N50
3.05E+04
LD50/ID50
3.05E+04
Dose Units
no of trophozoites
Response
death
Exposure Route
Swimming for 10 minutes
Contains Preferred Model
Status
fitted
a
0.241
Agent Strain
LEE
Experiment ID
956
Experiment Dataset
Description
Mice/Naegleria fowleri LEE strain data
Dose Dead Survived Total
1E+02 0 10 10
1E+03 0 10 10
1E+04 1 9 10
1E+05 4 6 10
1E+05 7 3 10

 

Goodness of fit and model selection
Model Deviance Δ Degrees 
of freedom

χ20.95,1

 

 χ20.95,m-k 
 
Exponential 6.13 5.88 4 3.84  9.49
Beta Poisson 0.25 3  7.81
Beta-Poisson is preferred to Exponential;
Beta-Poisson model plot with confidence bounds around optimized model
Beta-Poisson model plot with confidence bounds around optimized model
Host
CD1 Mice
# of Doses
5.00
Μodel
beta-Poisson
N50
1.99E+05
LD50/ID50
1.99E+05
Dose Units
no of trophozoites
Response
death
Exposure Route
Swimming 5 minutes
Contains Preferred Model
Status
fitted
a
0.353
Agent Strain
LEE
Experiment ID
955
Experiment Dataset
Description
Mice/Naegleria fowleri LEE strain data
Dose Dead Survived Total
1E+03 7 3 10
1E+04 8 2 10
1E+05 10 0 10
1E+06 10 0 10

 

Goodness of fit and model selection
Model Deviance Δ Degrees 
of freedom

χ20.95,1

 

 χ20.95,m-k 
 
Exponential 11.28 9.64 3 3.84 7.81 
0.325
Beta Poisson 1.64 2 5.99 
0.177
Beta-Poisson is preferred to Exponential;
Beta-Poisson model plot
Beta-Poisson model plot
# of Doses
4.00
Μodel
beta-Poisson
N50
422
LD50/ID50
422
Dose Units
no of trophozoites
Response
death
Exposure Route
intranasal
Contains Preferred Model
Status
fitted
a
0.536
Experiment ID
954
Host type
A/J mice
Experiment Dataset
Subscribe to no of trophozoites